3Q06
An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 85 |
Detector technology | CCD |
Collection date | 2009-04-27 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9340 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 164.591, 169.363, 55.336 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 3.200 |
R-factor | 0.2609 |
Rwork | 0.258 |
R-free | 0.31370 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 1TUP 1c26 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.241 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.720 | 3.370 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 23781 | |
<I/σ(I)> | 7.6 | 2.6 |
Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.15M DL-Malic Acid pH 7.0, 20% Polyethylene Glycol 3350, VAPOR DIFFUSION, HANGING DROP, temperature 277K |