3Q05
An induced fit mechanism regulates p53 DNA binding kinetics to confer sequence specificity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 85 |
| Detector technology | CCD |
| Collection date | 2009-02-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.87260 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 163.298, 169.764, 55.664 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.070 - 2.400 |
| R-factor | 0.2397 |
| Rwork | 0.238 |
| R-free | 0.26670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB entries 1TUP 1c26 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.201 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.070 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 59874 | |
| <I/σ(I)> | 10.9 | 2.5 |
| Completeness [%] | 98.6 | 98.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.15M DL-Malic Acid [pH 7.0], 20% Polyethylene Glycol 3350, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
