3PZL
The crystal structure of agmatine ureohydrolase of Thermoplasma volcanium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 95.139, 95.139, 198.375 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 82.390 - 2.700 |
| R-factor | 0.22208 |
| Rwork | 0.220 |
| R-free | 0.27105 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.373 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.400 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.124 | 0.680 |
| Number of reflections | 26146 | |
| <I/σ(I)> | 15.7 | 4.4 |
| Completeness [%] | 93.9 | 100 |
| Redundancy | 20.6 | 21.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9 | 291 | 0.1 M BICINE pH 9.0, 2% v/v 1,4-Dioxane, 10% w/v PEG 20,000, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
