3PZD
Structure of the myosin X MyTH4-FERM/DCC complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-01-01 |
| Detector | RIGAKU RAXIS |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 85.167, 49.528, 92.814 |
| Unit cell angles | 90.00, 112.58, 90.00 |
Refinement procedure
| Resolution | 29.580 - 2.500 |
| R-factor | 0.2056 |
| Rwork | 0.203 |
| R-free | 0.25890 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.145 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.2.25) |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.580 | 29.579 | 2.640 |
| High resolution limit [Å] | 2.500 | 7.910 | 2.500 |
| Rmerge | 0.073 | 0.027 | 0.468 |
| Rmeas | 0.032 | 0.547 | |
| Rpim | 0.018 | 0.281 | |
| Total number of observations | 2816 | 13214 | |
| Number of reflections | 24981 | ||
| <I/σ(I)> | 16.8 | 52.7 | 2.7 |
| Completeness [%] | 99.5 | 97.6 | 99.1 |
| Redundancy | 3.7 | 3.4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 8% PEG 8000, 10% glycerol, HEPES buffer, pH 7.5, vapor diffusion, hanging drop, temperature 289K |
