3PZ8

Crystal structure of Dvl1-DIX(Y17D) mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-14
Detector	RAYONIX MX-225
Wavelength(s)	0.9
Spacegroup name	I 21 21 21
Unit cell lengths	92.687, 106.780, 265.957
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.015 - 2.873
R-factor	0.2316
Rwork	0.230
R-free	0.26460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pz7
RMSD bond length	0.009
RMSD bond angle	1.193
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.015	2.950
High resolution limit [Å]	2.873	2.873
Rmerge	0.053	0.661
Number of reflections	30176
<I/σ(I)>	23.1	2.1
Completeness [%]	99.8	100
Redundancy	3.7	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	298	0.1M Hepes, 10% Ethylene Glycol, 0.66M Sodium Citrate, 0.3M Sodium Chloride, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K