3PXS
Crystal Structure of Diferrous MauG in Complex with Pre-Methylamine Dehydrogenase:
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-09 |
| Detector | MARMOSAIC 300 mm CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.530, 83.520, 107.780 |
| Unit cell angles | 109.94, 91.54, 105.78 |
Refinement procedure
| Resolution | 44.490 - 2.220 |
| R-factor | 0.18 |
| Rwork | 0.177 |
| R-free | 0.22900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.329 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | REFMAC (5.5.0109) |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.260 |
| High resolution limit [Å] | 2.220 | 2.220 |
| Rmerge | 0.085 | 0.343 |
| Number of reflections | 83223 | |
| <I/σ(I)> | 8.865 | 2.061 |
| Completeness [%] | 96.7 | 92.4 |
| Redundancy | 1.9 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.4 | 293 | 0.1M MES pH 6.4, 0.1M sodium acetate, 23-25 % w/v PEG 8000, vapor diffusion, hanging drop, temperature 293K , VAPOR DIFFUSION, HANGING DROP |
