3PXP
Crystal structure of a PAS and DNA binding domain containing protein (Caur_2278) from CHLOROFLEXUS AURANTIACUS J-10-FL at 2.30 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-23 |
| Detector | MAR MAR325 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 228.807, 83.568, 54.520 |
| Unit cell angles | 90.00, 103.12, 90.00 |
Refinement procedure
| Resolution | 29.750 - 2.300 |
| R-factor | 0.169 |
| Rwork | 0.168 |
| R-free | 0.20600 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.930 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | BUSTER-TNT (BUSTER 2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.748 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.059 | 0.318 |
| Number of reflections | 44327 | |
| <I/σ(I)> | 2.42 | 2.4 |
| Completeness [%] | 98.1 | 98.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.1 | 277 | 0.2M sodium chloride, 10.5% polyethylene glycol 8000, 0.1M CHES pH 9.1, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
