3PXN
Crystal structure of the Drosophila kinesin family member Kin10/NOD in complex with divalent manganese and ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2009-06-22 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.984 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.557, 75.712, 94.197 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.690 - 2.600 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3dc4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.760 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 10925 | |
| <I/σ(I)> | 24.5 | 4.3 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 7.1 | 7.22 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1 MES, 15% PEG 20000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
