3PXN

Crystal structure of the Drosophila kinesin family member Kin10/NOD in complex with divalent manganese and ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	77
Detector technology	CCD
Collection date	2009-06-22
Detector	ADSC QUANTUM 270
Wavelength(s)	0.984
Spacegroup name	P 21 21 21
Unit cell lengths	47.557, 75.712, 94.197
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.690 - 2.600
R-factor	0.227
Rwork	0.227
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dc4
RMSD bond length	0.008
RMSD bond angle	1.200
Data scaling software	XSCALE
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.760
High resolution limit [Å]	2.600	2.600
Number of reflections	10925
<I/σ(I)>	24.5	4.3
Completeness [%]	99.8	99.9
Redundancy	7.1	7.22

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	0.1 MES, 15% PEG 20000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K