3PXI
Structure of MecA108:ClpC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 141.806, 141.806, 656.078 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.153 - 6.926 |
| R-factor | 0.4081 |
| Rwork | 0.407 |
| R-free | 0.42190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pxg 1qvr |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.493 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_596)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.153 | 7.150 |
| High resolution limit [Å] | 6.926 | 6.900 |
| Rmerge | 0.074 | |
| Number of reflections | 7032 | |
| <I/σ(I)> | 26.31 | |
| Completeness [%] | 99.2 | 99.4 |
| Redundancy | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 291 | 10% PEG 3350, 400mM magnesium formate, 100mM MES, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
