3PXG

Structure of MecA121 and ClpC1-485 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	137.560, 137.560, 445.650
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.524 - 3.654
R-factor	0.26
Rwork	0.257
R-free	0.30300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2y1r 1qvr
RMSD bond length	0.114
RMSD bond angle	1.985
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.524	3.780
High resolution limit [Å]	3.654	3.650
Rmerge	0.081	0.732
Number of reflections	53303
<I/σ(I)>	19.34	1.64
Completeness [%]	96.1	76.5
Redundancy	5.2	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	12% PEG 3350, 450mM sodium malonate, 100mM MES, pH7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K