3PVS
Structure and biochemical activities of Escherichia coli MgsA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2009-07-24 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 99.946, 143.689, 163.186 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.1984 |
| Rwork | 0.195 |
| R-free | 0.26600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.608 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 6.360 | 2.350 |
| Rmerge | 0.068 | 0.025 | 0.494 |
| Number of reflections | 89807 | ||
| <I/σ(I)> | 8.2 | ||
| Completeness [%] | 91.3 | 99.6 | 54.5 |
| Redundancy | 5.8 | 6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 298 | 10 mM MES, pH 6.6, 38% Ethylene Glycol, 16% Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
