3PVH

Structural and Functional Analysis of Arabidopsis thaliana thylakoid lumen protein AtTLP18.3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-06-26
Detector	ADSC QUANTUM 210r
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	46.871, 49.890, 76.511
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.440 - 1.600
Rwork	0.195
R-free	0.23000
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3ptj
RMSD bond length	0.022
RMSD bond angle	2.200
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.660
High resolution limit [Å]	1.600	3.450	1.600
Rmerge	0.035	0.031	0.316
Number of reflections	24243
<I/σ(I)>	58.6	55	8.7
Completeness [%]	99.3	93.6	100
Redundancy	14.2	12.3	14.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	296	0.2M Sodium acetate trihydrate, 0.1M Sodium cacodylate (pH 6.5), 25-30% (w/v) Polyethylene glycol 4000 , VAPOR DIFFUSION, temperature 296K