3PVE
Crystal structure of the G2 domain of Agrin from Mus Musculus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-19 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.620, 45.580, 51.510 |
| Unit cell angles | 72.28, 68.53, 65.44 |
Refinement procedure
| Resolution | 36.380 - 1.400 |
| R-factor | 0.194 |
| Rwork | 0.192 |
| R-free | 0.22890 |
| Structure solution method | SAD |
| Starting model (for MR) | ARP/wARP |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.689 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX (C) |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.160 | 1.480 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 57584 | |
| <I/σ(I)> | 7.6 | 2.3 |
| Completeness [%] | 93.3 | 92.6 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 294 | 100mM sodium acetate, 15% PEG 20000, pH 4.6, Vapor diffusion, sitting drop, temperature 294K |
