3PV1

Crystal structure of the USP15 DUSP-UBL domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	C 2 2 21
Unit cell lengths	96.660, 122.980, 105.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.938 - 2.600
R-factor	0.2085
Rwork	0.205
R-free	0.27400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.084
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	53.070	53.070	2.740
High resolution limit [Å]	2.600	2.740	2.600
Rmerge		0.099	0.740
Number of reflections	19389
<I/σ(I)>		3.7	1.7
Completeness [%]	99.2	96.5	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	292	18% PEG 4000, 0.1M Tris, 0.1M magnesium acetate, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 292K