3PUV
Crystal Structure of an outward-facing MBP-Maltose transporter complex bound to ADP-VO4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-10 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97965 |
| Spacegroup name | P 1 |
| Unit cell lengths | 81.786, 97.125, 112.791 |
| Unit cell angles | 85.58, 78.71, 72.69 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.2243 |
| Rwork | 0.223 |
| R-free | 0.25335 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2r6g |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.936 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.071 | |
| Number of reflections | 77414 | |
| <I/σ(I)> | 2.1 | |
| Completeness [%] | 58.5 | 14.7 |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 27% PEG 400, 0.1M HEPES, 10 mM magnesium chloride, 50 mM sodium chloride, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 7.5 |
