3PUG
Haloferax volcanii Malate Synthase Native at 3mM Glyoxylate
Experimental procedure
| Experimental method | SIRAS |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-03-18 |
| Detector | RIGAKU |
| Wavelength(s) | 1.54 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 156.370, 156.370, 141.455 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.700 |
| R-factor | 0.2016 |
| Rwork | 0.198 |
| R-free | 0.26260 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.014 |
| RMSD bond angle | 2.273 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE (2.13) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 5.800 | 2.700 |
| Rmerge | 0.090 | 0.048 | 0.383 |
| Number of reflections | 18255 | ||
| <I/σ(I)> | 15.6 | ||
| Completeness [%] | 100.0 | 99.8 | 100 |
| Redundancy | 5.7 | 5.5 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.4 | 295 | PEG 4500, glycerol, 2M KCl,13 mM MgCl2, 3mM Glyoxylate, pH 4.4, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
