3PU2
Crystal Structure of the Q3J4M4_RHOS4 protein from Rhodobacter sphaeroides. Northeast Structural Genomics Consortium Target RhR263.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-29 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 75.214, 35.856, 230.468 |
| Unit cell angles | 90.00, 98.98, 90.00 |
Refinement procedure
| Resolution | 29.097 - 2.606 |
| R-factor | 0.231 |
| Rwork | 0.230 |
| R-free | 0.26300 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.517 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.6_289) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.064 | 0.263 |
| Number of reflections | 68098 | |
| <I/σ(I)> | 12.9 | 2.3 |
| Completeness [%] | 94.3 | 72.3 |
| Redundancy | 1.7 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch under paraffin oil | 6 | 291 | 24% PEG 20000, 0.1M potassium bromide, 0.1M MES, pH 6.0, microbatch under paraffin oil, temperature 291K |
