3PTO

Crystal Structure of an empty Vesicular Stomatitis Virus Nucleocapsid Protein Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	293
Collection date	2009-06-05
Detector	MAR 325 CCD
Wavelength(s)	0.97946
Spacegroup name	P 21 21 2
Unit cell lengths	165.643, 234.062, 75.975
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.763 - 3.008
R-factor	0.2592
Rwork	0.258
R-free	0.29070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.375
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.6.1_357)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.071	0.320
Number of reflections	51615
<I/σ(I)>		2.4
Completeness [%]	86.7	39.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	293	5.5 % PEG 3350, 200 mM sodium chloride, 100mM sodium acetate, pH 4.5, vapor diffusion, hanging drop, temperature 293.0K