3PTH
The PABC1 MLLE domain bound to the variant PAM2 motif of LARP4B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-14 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 29.150, 57.194, 59.514 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.240 - 1.700 |
| R-factor | 0.1735 |
| Rwork | 0.172 |
| R-free | 0.21786 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.607 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 41.240 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 11136 |
| <I/σ(I)> | 14.8 |
| Completeness [%] | 96.9 |
| Redundancy | 3.68 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 289 | 2 ul protein solution at 43 mg/ml containing the 1.5 fold molar amount of peptide ligand was mixed with 2ul reservoir solution containing 1.5M magnesium sulfate, pH 6.5, VAPOR DIFFUSION, temperature 289K |
