3PTE

THE REFINED CRYSTALLOGRAPHIC STRUCTURE OF A DD-PEPTIDASE PENICILLIN-TARGET ENZYME AT 1.6 A RESOLUTION

Replaces:  1PTE

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	51.060, 67.310, 102.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.600
R-factor	0.148
Rwork	0.148
R-free	0.21100
RMSD bond length	0.020
RMSD bond angle	0.035 *
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	1.600 *
Rmerge	0.158 *
Number of reflections	41176
Completeness [%]	88.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	20 (mg/ml)
2	1	1	sodium phosphate	50 (mM)
3	1	1	PEG8000	15 (%(w/v))
4	1	2	PEG8000	20 (%)