3PT1
Structure of DUF89 from Saccharomyces cerevisiae co-crystallized with F6P.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-06-30 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 53.320, 108.650, 88.080 |
| Unit cell angles | 90.00, 96.84, 90.00 |
Refinement procedure
| Resolution | 29.891 - 1.773 |
| R-factor | 0.158 |
| Rwork | 0.156 |
| R-free | 0.19150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.040 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.070 | 1.870 |
| High resolution limit [Å] | 1.770 | 1.770 |
| Rmerge | 0.048 | 0.230 |
| Number of reflections | 48080 | |
| <I/σ(I)> | 19.8 | 5.5 |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.1M Bis-Tris, 0.2M MgCl2, 25% PEG3350, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
