3PSZ
Crystal Structure of AhQnr, the Qnr protein from Aeromonas hydrophila (P21212 crystal form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.117 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 88.730, 116.290, 55.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.450 - 2.200 |
| R-factor | 0.21379 |
| Rwork | 0.211 |
| R-free | 0.26990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pss |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.364 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.430 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.132 | 0.424 |
| Number of reflections | 28777 | |
| <I/σ(I)> | 8 | 3.1 |
| Completeness [%] | 98.1 | 98.6 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 291 | 0.1M sodium cacodylate, 1.5M sodium acetate, 20mM DTT, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
