3PSJ

Crystal Structure of the Spt6 Tandem SH2 Domain from Saccharomyces cerevisiae, Form Se-Spt6 (1247-1451)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-1
Synchrotron site	SSRL
Beamline	BL9-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-06-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97908
Spacegroup name	I 41 2 2
Unit cell lengths	98.247, 98.247, 131.927
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.784 - 2.702
R-factor	0.2116
Rwork	0.207
R-free	0.25440
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.117
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	2.800
High resolution limit [Å]	2.700	5.810	2.700
Rmerge	0.070	0.038	0.296
Number of reflections	15594
<I/σ(I)>	14.5
Completeness [%]	92.2	93.5	74.5
Redundancy	6.9	8.1	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	294	22% PEG 4000, 0.3M Ammonium Sulfate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K