3PSF

Crystal Structure of the Spt6 core domain from Saccharomyces cerevisiae, Form Spt6(236-1259)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-08
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97922
Spacegroup name	P 21 21 21
Unit cell lengths	115.052, 116.176, 117.348
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.320 - 2.590
R-factor	0.2255
Rwork	0.224
R-free	0.26510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.895
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (1.3.3)
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall
Low resolution limit [Å]	32.320
High resolution limit [Å]	2.590
Number of reflections	48005
Completeness [%]	96.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	286	50mM Mg(HCO2)2, 10% Glycerol, 1.0 M NDSB-256 , pH 7.6, VAPOR DIFFUSION, SITTING DROP, temperature 286K