3PS7
Biochemical studies and crystal structure determination of dihydrodipicolinate synthase from Pseudomonas aeruginosa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 283 |
Detector technology | IMAGE PLATE |
Collection date | 2009-09-28 |
Detector | MARRESEARCH |
Wavelength(s) | 1.514 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.070, 81.590, 123.690 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.570 - 2.850 |
R-factor | 0.20677 |
Rwork | 0.205 |
R-free | 0.24160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dhp |
RMSD bond length | 0.015 |
RMSD bond angle | 1.650 |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.570 | 3.000 |
High resolution limit [Å] | 2.850 | 2.850 |
Number of reflections | 14342 | |
Completeness [%] | 96.4 | 98.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 30% PEG 3350, 170mM MgCl2, 70mM Tris-HCl, 6% propylene glycol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |