3PS7

Biochemical studies and crystal structure determination of dihydrodipicolinate synthase from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	283
Detector technology	IMAGE PLATE
Collection date	2009-09-28
Detector	MARRESEARCH
Wavelength(s)	1.514
Spacegroup name	P 21 21 21
Unit cell lengths	58.070, 81.590, 123.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.570 - 2.850
R-factor	0.20677
Rwork	0.205
R-free	0.24160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dhp
RMSD bond length	0.015
RMSD bond angle	1.650
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.570	3.000
High resolution limit [Å]	2.850	2.850
Number of reflections	14342
Completeness [%]	96.4	98.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	30% PEG 3350, 170mM MgCl2, 70mM Tris-HCl, 6% propylene glycol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K