3PS4
PDZ domain from Human microtubule-associated serine/threonine-protein kinase 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97630 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 62.100, 79.326, 82.856 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.480 - 1.850 |
| R-factor | 0.1956 |
| Rwork | 0.194 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v90 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.080 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.260 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.066 | 0.731 |
| Number of reflections | 35510 | |
| <I/σ(I)> | 12.8 | 2.2 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 4.1 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.1 | 277 | 25 w/v PEG_MME_2000 85 mM Imidazole pH 6.1, 15 mM Imidazole pH 8.1, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
