3PRX
Structure of Complement C5 in Complex with CVF and SSL7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-18 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 163.650, 181.960, 392.780 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.214 - 4.300 |
| R-factor | 0.2091 |
| Rwork | 0.208 |
| R-free | 0.26140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3KLS and 3HRZ |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.584 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.214 | |
| High resolution limit [Å] | 4.300 | 4.300 |
| Number of reflections | 77924 | |
| <I/σ(I)> | 11.4 | 3.7 |
| Completeness [%] | 96.8 | 98.6 |
| Redundancy | 5.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.4 | 292 | 3 M sodium malonate, pH 7.4, VAPOR DIFFUSION, temperature 292K |
