3PRW

Crystal structure of the lipoprotein BamB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-08-13
Detector	PSI PILATUS 6M
Wavelength(s)	0.96
Spacegroup name	I 2 2 2
Unit cell lengths	50.221, 120.967, 134.117
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9.995 - 1.800
R-factor	0.1844
Rwork	0.183
R-free	0.20440
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.145
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHARP
Refinement software	PHENIX (1.6_289)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.300	17.300
High resolution limit [Å]	1.800	1.800
Rmerge	0.049	0.079
Number of reflections	37883
<I/σ(I)>	27.4	16.7
Completeness [%]	99.5	99.6
Redundancy	6	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.9	292	100 mM Tris, pH 6.9, 20% PEG 3350, 200 mM ammonium citrate, VAPOR DIFFUSION, SITTING DROP, temperature 292K