3PRL
Crystal structure of NADP-dependent glyceraldehyde-3-phosphate dehydrogenase from Bacillus halodurans C-125
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.302, 131.430, 83.891 |
| Unit cell angles | 90.00, 102.95, 90.00 |
Refinement procedure
| Resolution | 19.980 - 2.000 |
| R-factor | 0.2512 |
| Rwork | 0.248 |
| R-free | 0.30630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1euh |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.394 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
| Rmerge | 0.107 | 0.049 | 0.684 |
| Number of reflections | 123229 | ||
| <I/σ(I)> | 7.4 | ||
| Completeness [%] | 99.7 | 99.7 | 99.7 |
| Redundancy | 5.2 | 5.6 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.2 M ammonium sulfate, 0.1 M Bis-Tris, 25% PEG 3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
