3PRH
tryptophanyl-tRNA synthetase Val144Pro mutant from B. subtilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-24 |
| Detector | ADSC |
| Wavelength(s) | 0.9805 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.258, 119.816, 127.353 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.950 - 2.800 |
| R-factor | 0.23714 |
| Rwork | 0.235 |
| R-free | 0.27504 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3FIO |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.314 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.050 | 0.300 |
| Number of reflections | 19307 | |
| <I/σ(I)> | 13.5 | 2.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.1 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.05 M MgCl2, 0.05 M HEPES/NaOH pH 7, 25% PEG550, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
