3PRB

Structural analysis of protein folding by the Methanococcus jannaschii chaperone FKBP26

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2000-07-01
Spacegroup name	P 21 21 21
Unit cell lengths	55.532, 65.199, 148.616
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.840 - 2.200
R-factor	0.2289
Rwork	0.227
R-free	0.27360
Structure solution method	MAD
RMSD bond length	0.013
RMSD bond angle	1.283
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.02)
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	19.840
High resolution limit [Å]	2.200
Number of reflections	25822

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	hanging drop		298	15% PMME550, 100NaCl, pH 100 mM BICINE, pH 9.0, hanging drop, temperature 298K
1	hanging drop		298	15% PMME550, 100NaCl, pH 100 mM BICINE, pH 9.0, hanging drop, temperature 298K