3PR6
Crystal structure analysis of yeast TRAPP associate protein Tca17
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-12 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9796,0.9798,0.9720 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 57.130, 58.492, 53.969 |
| Unit cell angles | 90.00, 92.62, 90.00 |
Refinement procedure
| Resolution | 29.250 - 1.800 |
| R-factor | 0.1851 |
| Rwork | 0.183 |
| R-free | 0.21960 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.277 |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 29.250 | 1.850 | |
| High resolution limit [Å] | 1.800 | 8.050 | 1.800 |
| Rmerge | 0.039 | 0.039 | 0.408 |
| Number of reflections | 16453 | 180 | 1216 |
| <I/σ(I)> | 17.49 | 37.8 | 3 |
| Completeness [%] | 99.4 | 90.5 | 99.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 25% PEG3350, 0.2 M magnesium chloride, 0.1 M Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
