3PQZ
Grb7 SH2 with peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-01 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.660, 79.110, 54.690 |
| Unit cell angles | 90.00, 104.40, 90.00 |
Refinement procedure
| Resolution | 32.882 - 2.413 |
| R-factor | 0.2396 |
| Rwork | 0.238 |
| R-free | 0.27740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.205 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 34.000 | 34.000 | 2.620 |
| High resolution limit [Å] | 2.400 | 5.400 | 2.410 |
| Number of reflections | 15617 | ||
| Completeness [%] | 93.1 | 91 | 92 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 293 | 17.5% PEG3350, 0.1M Glycine, 10% Glycerol, pH 6.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
