3PQU
The crystal structures of porcine pathogen AsH57_TbpB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Detector technology | CCD |
| Collection date | 2009-12-17 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.670, 74.470, 106.400 |
| Unit cell angles | 90.00, 105.70, 90.00 |
Refinement procedure
| Resolution | 44.755 - 2.100 |
| R-factor | 0.1744 |
| Rwork | 0.172 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hol |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.071 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | PHENIX (1.5_2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.890 |
| High resolution limit [Å] | 1.820 | 3.920 | 1.820 |
| Rmerge | 0.088 | 0.054 | |
| Number of reflections | 79607 | ||
| <I/σ(I)> | 8 | ||
| Completeness [%] | 63.4 | 99.8 | 1.8 |
| Redundancy | 3.7 | 5.1 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1 M Bis-Tris, 0.4 M lithium sulfate, 17% PEG 3350, 20% glycerol, pH 5.5, vapor diffusion, hanging drop, temperature 293K |
