3PQH
Crystal structure of the C-terminal fragment of the bacteriophage phi92 membrane-piercing protein gp138
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-05-07 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 48.080, 48.080, 553.203 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.521 - 1.295 |
| R-factor | 0.1247 |
| Rwork | 0.123 |
| R-free | 0.15980 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.751 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX (C/D/E) |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.520 | 1.370 |
| High resolution limit [Å] | 1.290 | 1.290 |
| Rmerge | 0.087 | 0.811 |
| Number of reflections | 61401 | |
| <I/σ(I)> | 17.6 | |
| Completeness [%] | 98.6 | 98.1 |
| Redundancy | 16.6 | 15.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 7mg/ml 34% - 38% PEE 100mM - 400mM KCl 100mM MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
