3PPT

REP1-NXSQ fatty acid transporter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-04
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	40.322, 49.599, 60.492
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.250 - 1.280
R-factor	0.136
Rwork	0.134
R-free	0.17100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yiv
RMSD bond length	0.008
RMSD bond angle	1.291
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.330
High resolution limit [Å]	1.280	1.280
Number of reflections	31531
<I/σ(I)>	32.8	4.1
Completeness [%]	98.7	97.6
Redundancy	5.6	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5	277	35% PEG 2000 MME, 0.2 M KCL, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K