3PPS
Crystal structure of an ascomycete fungal laccase from Thielavia arenaria
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-22 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.360, 178.950, 118.130 |
| Unit cell angles | 90.00, 90.26, 90.00 |
Refinement procedure
| Resolution | 42.650 - 2.500 |
| R-factor | 0.1839 |
| Rwork | 0.182 |
| R-free | 0.22220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.776 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.800 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 87791 | |
| Completeness [%] | 99.9 | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 294 | 7.5% PEG 3350, 0.2 M ammonium sulfate and 0.1 M sodium acetate in pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
