3POU

Crystal structure of E.coli OmpF porin in lipidic cubic phase: space group H32, large unit cell

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-20
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.00
Spacegroup name	H 3 2
Unit cell lengths	85.710, 85.710, 332.941
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.990 - 2.800
R-factor	0.1982
Rwork	0.196
R-free	0.25150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2omf
RMSD bond length	0.015
RMSD bond angle	1.646
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	19.990	19.989	2.950
High resolution limit [Å]	2.800	8.860	2.800
Rmerge		0.014	0.014
Rmeas		0.087	1.524
Rpim		0.032	0.492
Total number of observations		2876	16366
Number of reflections	12017
<I/σ(I)>	6.8	12.5	2
Completeness [%]	99.7	91.4	100
Redundancy	9	7.2	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.6	296	0.1M sodium citrate, 0.7M sodium tartrate, 1.0M potassium thiocyanate, pH 5.6, lipidic sponge phase, temperature 296K