3POS
Crystal structure of the globular domain of human calreticulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97925 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.230, 91.100, 194.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.940 - 1.650 |
| R-factor | 0.1715 |
| Rwork | 0.170 |
| R-free | 0.20470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AMoRE |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.700 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.117 | 0.454 |
| Number of reflections | 88382 | |
| <I/σ(I)> | 18.81 | 2.4 |
| Completeness [%] | 96.8 | 83 |
| Redundancy | 16 | 13 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.1 | 30% PEG 4000, 0.1M sodium acetate, 0.1M Tris, pH 8.5-9, VAPOR DIFFUSION, HANGING DROP, temperature 277.1K |
