3POR

PORIN CONFORMATION IN THE ABSENCE OF CALCIUM; REFINED STRUCTURE AT 2.5 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	H 3
Unit cell lengths	95.300, 95.300, 146.800
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 2.500
R-factor	0.188 *
Rwork	0.188
RMSD bond length	0.022
RMSD bond angle	27.900 *
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	9999.000 *
High resolution limit [Å]	2.500 *
Rmerge	0.052 *
Number of reflections	16568 *
Completeness [%]	95.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5-7 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	drop		300 (mM)
4	1	drop		3 (mM)
5	1	drop	n-octyltetraoxyethylene	0.6 (%(w/v))
6	1	drop	PEG600	7-10 (%(w/v))
7	1	reservoir	PEG600	23-30 (%(w/v))