3POM
Crystal Structure of the Unliganded Retinoblastoma Protein Pocket Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979464 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.282, 111.164, 138.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.500 - 2.500 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.466 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.300 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 33106 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10.6 | 277 | 100 mM CAPS, 10% PEG 3350, pH 10.6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
