3POK
Interleukin-1-beta LBT L3 Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-01-01 |
| Detector | RIGAKU RAXIS IV++ |
| Spacegroup name | P 41 |
| Unit cell lengths | 42.595, 42.595, 88.002 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.170 - 1.700 |
| R-factor | 0.1787 |
| Rwork | 0.175 |
| R-free | 0.21160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.145 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.200 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 33108 | |
| <I/σ(I)> | 4.77 | |
| Completeness [%] | 97.4 | 91 |
| Redundancy | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 100mM sodium acetate trihydrate pH 4.5, 3.0 M NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
