3PNY
Structure of Glutamyl-tRNA synthetase from Mycobacterium tuberculosis in space group P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-12 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.159, 210.553, 58.145 |
| Unit cell angles | 90.00, 99.80, 90.00 |
Refinement procedure
| Resolution | 10.410 - 1.700 |
| R-factor | 0.2446 |
| Rwork | 0.242 |
| R-free | 0.28499 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ja2 |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.425 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.720 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.079 | 0.266 |
| Number of reflections | 98097 | |
| <I/σ(I)> | 16.7 | 3.7 |
| Completeness [%] | 90.6 | 94.8 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 200mM Ammonium Acetate, 15% (W/V)PEG4000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
