3PNV
V369M mutant of Glutamyl-tRNA synthetase from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-15 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.434, 210.346, 57.979 |
| Unit cell angles | 90.00, 99.35, 90.00 |
Refinement procedure
| Resolution | 10.410 - 1.950 |
| R-factor | 0.27464 |
| Rwork | 0.272 |
| R-free | 0.31578 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ja2 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.301 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.052 | 0.529 |
| Number of reflections | 69795 | |
| <I/σ(I)> | 20.2 | 2.1 |
| Completeness [%] | 96.9 | 98.5 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 200mM Ammonium Acetate, 15% (W/V)PEG4000, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
