3PNK
Crystal Structure of E.coli Dha kinase DhaK
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-15 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.833, 91.507, 73.232 |
| Unit cell angles | 90.00, 89.96, 90.00 |
Refinement procedure
| Resolution | 49.830 - 2.210 |
| R-factor | 0.1738 |
| Rwork | 0.172 |
| R-free | 0.20990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1oi2 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.354 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.830 | 50.000 | 2.290 |
| High resolution limit [Å] | 2.210 | 4.760 | 2.210 |
| Rmerge | 0.122 | 0.073 | 0.457 |
| Number of reflections | 30618 | ||
| <I/σ(I)> | 9.5 | ||
| Completeness [%] | 93.3 | 99.7 | 81.1 |
| Redundancy | 5.2 | 6.4 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 295 | 20% PEG 8000, 0.1M Sodium Citrate pH 5.6, vapor diffusion, sitting drop, temperature 295K |
