3PN9
Crystal structure of a proline dipeptidase from streptococcus pneumoniae tigr4
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-31 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97940, 0.97937 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 118.073, 118.073, 185.348 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 99.580 - 2.000 |
| R-factor | 0.184 |
| Rwork | 0.183 |
| R-free | 0.20400 |
| Structure solution method | MAD |
| RMSD bond length | 0.037 |
| RMSD bond angle | 2.493 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.580 | 2.020 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.091 | 0.800 |
| Number of reflections | 168476 | |
| <I/σ(I)> | 38.209 | 2.8 |
| Completeness [%] | 100.0 | 94.1 |
| Redundancy | 9.9 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | Tris(0.1M, PH=8.5), LITHIUM SULFATE (0.2M, PH=N/A), AMMONIUM SULFATE (2M, PH=N/A), TEMPERATURE 277K, VAPOR DIFFUSION, SITTING DROP |
