3PMV
Ligand-binding domain of GluA2 (flip) ionotropic glutamate receptor in complex with an allosteric modulator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-03-14 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 64.220, 87.230, 47.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.610 - 1.800 |
| R-factor | 0.18716 |
| Rwork | 0.184 |
| R-free | 0.25232 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3o28 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.613 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.610 | 1.860 |
| High resolution limit [Å] | 1.790 | 1.800 |
| Rmerge | 0.050 | 0.287 |
| Number of reflections | 24891 | |
| <I/σ(I)> | 13.2 | 3.5 |
| Completeness [%] | 98.1 | 82.9 |
| Redundancy | 4.1 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 277 | 18% PEG 4000, 50mM Lithium Sulphate, 2.5% Glycerol, 100mM Sodium Cacodylate pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
