3PMG

STRUCTURE OF RABBIT MUSCLE PHOSPHOGLUCOMUTASE AT 2.4 ANGSTROMS RESOLUTION. USE OF FREEZING POINT DEPRESSANT AND REDUCED TEMPERATURE TO ENHANCE DIFFRACTIVITY

Replaces:  2PMG

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1993-10
Detector	XUONG-HAMLIN MULTIWIRE
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	174.420, 174.420, 101.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.400
R-factor	0.163
Rwork	0.163
R-free	0.19100
RMSD bond length	0.018
RMSD bond angle	24.100 *
Data reduction software	XTS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.350 *	2.350 *
Rmerge	0.054
Number of reflections	54817 *
Completeness [%]	84.2 *	56.8 *
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG600	55 (%)
2	1	reservoir	MES	10 (mM)
3	1	reservoir		1 (mM)