3PLW
Ref protein from P1 bacteriophage
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2010-02-18 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 71.733, 71.733, 54.236 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 1.400 |
| R-factor | 0.1637 |
| Rwork | 0.163 |
| R-free | 0.17170 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.183 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | RESOLVE (2.15) |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.420 |
| High resolution limit [Å] | 1.400 | 3.800 | 1.400 |
| Rmerge | 0.086 | 0.048 | 0.435 |
| Number of reflections | 30636 | ||
| <I/σ(I)> | 10 | ||
| Completeness [%] | 95.9 | 98.8 | 69.4 |
| Redundancy | 13.1 | 13.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 0.2 M ammonium nitrate, 20% PEG3350, 0.01 M Tris-HCl, pH 8.0, 100 mM sodium chloride, VAPOR DIFFUSION, HANGING DROP |
