3PLU
Structure of Hub-1 protein in complex with Snu66 peptide (HINDI)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2006-05-09 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 1 |
| Unit cell lengths | 35.230, 36.340, 36.780 |
| Unit cell angles | 83.44, 89.85, 85.84 |
Refinement procedure
| Resolution | 26.120 - 1.400 |
| R-factor | 0.18737 |
| Rwork | 0.185 |
| R-free | 0.22819 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubi |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.033 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.500 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.318 | |
| Number of reflections | 33677 | |
| <I/σ(I)> | 5.56 | |
| Completeness [%] | 94.6 | 92.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.9 | 290 | 20% PEG 3350, 0.2M Ammonium Chloride, pH 6.9, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
